Optical clearing techniques have emerged as pivotal methods in biomedical imaging by reducing the inherent light scattering in biological tissues. By modulating the optical properties of tissue – ...
Steps for deep-tissue 3D-imaging. Embryos or neonates fixed with 4% PFA were optically cleared with 60% TDE or tissue-clearing reagent CUBIC-L/R, and imaged using stereoscopic, confocal, or two-photon ...
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