Transfer the sections onto glass slides suitable for immunohistochemistry. Allow the slides to dry overnight and store slides at room temperature until ready for use.

Sample preparation for IHC may include fixation, dehydration, embedding, and sectioning, but one or two experimental variables usually determine the exact workflow.

My Antibody Suggestions These are the IHC approved antibodies that I have optimized for staining in the Oliver Lab. Some of them are ideal and others may need further optimization. I have indicated …

Critical steps in the recommended protocol – how critical are they? can have a significant effect on IHC results. We have tested common variations in antigen retrieval buffers, boiling devices, antibody …

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

In an effort to accelerate your immunostaining of tissue sections and cell climbing slices, we have developed and validated our step-by-step IHC/ICC/IF protocols to cover biospecimen preparation …

This step by step protocol provides everything you need to carry out successful immunohistochemistry.

Our staining kits are provided with complete protocols for use. This page lists the steps involved in using selected Histostain™ kits and the time required for each step.

The Immunochemistry staff supplies protocols for the preparation of samples. Sort by name, medium, fixative, species, or staining. Use the live search to filter out protocols.

Preparing fixed tissues for frozen sectioning Below is a general protocol for fixing tissues before sectioning on the cryostat.