In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can be easily …

Improve accuracy, increase efficiency and reduce errors with this handy guide to the plasmid cloning cycle.

Digest plasmid with the appropriate restriction enzymes to produce a DNA fragment that can be cloned directly into a vector. Unidirectional cloning is achieved with restriction enzymes that …

Often, the first step in a molecular biology experiment is to clone (i.e. copy) a gene into a plasmid, then transform this recombinant plasmid back into bacteria so that essentially unlimited copies …

In a typical cloning experiment, a target gene is inserted into a circular piece of DNA called a plasmid. The plasmid is introduced into bacteria via a process called transformation, and …

Explore the intricate process of DNA cloning using plasmid vectors. Learn about methodologies, applications, challenges, and future prospects in genetic research! 🧬🔬

Plasmids are a type of cloning vector. A vector is used to introduce DNA into cells. There are many types of vector, which is a more general term, and plasmids (the more specific term) are …

5 days ago · Molecular cloning is the process by which we construct synthetic DNA sequences, aka how we build plasmids in lab. (Since, alas, we do not have cheap, multi-kilobase-scale …

Vectors used in traditional cloning methods are based on plasmids, which are double-stranded, circular DNAs that replicate inside bacteria independently of the genomic DNA.

With molecular cloning, scientists can amplify and manipulate genes of interest and then insert them into plasmids for replication and protein expression. There are many methods that allow …

Plasmid vectors allow the DNA of interest to be copied easily in large amounts, and often provide the necessary control elements to be used to direct transcription and translation of the cloned …

May 27, 2025 · Plasmid cloning is a cornerstone of genetic engineering and has numerous applications. It is used in the production of recombinant proteins, such as insulin and growth …

Plasmids occupy a place of honor in molecular cloning: They were used in the first recombinant DNA experiments and, 40 or more years later, they remain as the carriage horses of molecular …

Jul 23, 2025 · DNA cloning involves isolating a DNA fragment of interest and inserting it into a vector, such as a plasmid or a bacteriophage, to produce a recombinant DNA molecule.

Watch this in-depth video series and discover the key concepts of molecular cloning and the plasmid cloning cycle.

We store and distribute high-quality plasmids from your colleagues. A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and …

Nov 1, 2021 · Plasmids occupy a place of honor in molecular cloning: They were used in the first recombinant DNA experiments and, 40 or more years later, they remain as the carriage horses …

Multiple cloning sites are strategically designed to maximize flexibility while maintaining vector integrity. The primary consideration is the inclusion of unique restriction enzyme recognition …

The insertion of foreign DNA fragments into the plasmid via molecular cloning techniques is fundamental to the functionality of plasmids. The inserted fragments may include target genes, …

Mar 29, 2016 · In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can be …

Plasmid DNA Plasmids are small, circular, double-stranded DNA used in molecular biology for manipulating and decoding genetic information. They have evolved as key components in any …

In this section of the blog, learn more about the different parts of a plasmid, molecular cloning techniques, tips for using plasmids, specific plasmid-based tools, and more.